Generation of Defective Interfering RNA Dimers of Cymbidium Ringspot Tombusvirus
Identifieur interne : 004180 ( Main/Exploration ); précédent : 004179; suivant : 004181Generation of Defective Interfering RNA Dimers of Cymbidium Ringspot Tombusvirus
Auteurs : Tamás Dalmay [Hongrie] ; György Szittya [Hongrie] ; J Zsef Burgyán [Hongrie]Source :
- Virology [ 0042-6822 ] ; 1995.
Abstract
Abstract: Inoculation of Nicotiana clevelandii and N. benthamiana plants with in vitro transcripts of both genomic and defective interfering (DI) RNAs of cymbidium ringspot tombusvirus resulted in a rapid accumulation of new DI-like RNA species which were demonstrated by cloning and sequencing to be head-to-tail dimers of unit length DI RNAs. The junction regions of dimers were represented by sequences derived precisely from the 5′ and 3′ termini of DI RNAs. Only infection with DI RNAs of smaller size (DI-2 and DI-3, 402 and 482 nt, respectively) produced detectable amount of dimers; in contrast, infection with the largest DI RNA (DI-13, 679 nt) was unable to accumulate dimers during viral infection. Analysis of mutant DI RNAs containing deletions or insertions revealed that the size of the monomer molecule is a major factor in the accumulation of dimers. Monomeric DI RNAs were formed in both plants and protoplasts inoculated with in vitro-transcribed directs. No heterodimers were found in plants inoculated simultaneously with DI-2 and DI-3 RNA molecules, which may indicate that replicase is not released from the template during synthesis of dimer molecules. However, the occurrence of a recombinant DI RNA dimer molecule derived from the two DI RNAs suggests that simultaneous infection of the same cells with two DI RNAs did indeed take place and that absence of heterodimers did not depend on compartmentalization.
Url:
DOI: 10.1006/viro.1995.1111
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001744
- to stream Istex, to step Curation: 001744
- to stream Istex, to step Checkpoint: 001871
- to stream Main, to step Merge: 004241
- to stream Main, to step Curation: 004180
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Generation of Defective Interfering RNA Dimers of Cymbidium Ringspot Tombusvirus</title>
<author><name sortKey="Dalmay, Tamas" sort="Dalmay, Tamas" uniqKey="Dalmay T" first="Tamás" last="Dalmay">Tamás Dalmay</name>
</author>
<author><name sortKey="Szittya, Gyorgy" sort="Szittya, Gyorgy" uniqKey="Szittya G" first="György" last="Szittya">György Szittya</name>
</author>
<author><name sortKey="Burgyan, J Zsef" sort="Burgyan, J Zsef" uniqKey="Burgyan J" first="J Zsef" last="Burgyán">J Zsef Burgyán</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:4F7A384318A00A102D81113FBDC4101FF1F267F9</idno>
<date when="1995" year="1995">1995</date>
<idno type="doi">10.1006/viro.1995.1111</idno>
<idno type="url">https://api.istex.fr/ark:/67375/6H6-KZ9KH66D-L/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001744</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001744</idno>
<idno type="wicri:Area/Istex/Curation">001744</idno>
<idno type="wicri:Area/Istex/Checkpoint">001871</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001871</idno>
<idno type="wicri:doubleKey">0042-6822:1995:Dalmay T:generation:of:defective</idno>
<idno type="wicri:Area/Main/Merge">004241</idno>
<idno type="wicri:Area/Main/Curation">004180</idno>
<idno type="wicri:Area/Main/Exploration">004180</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Generation of Defective Interfering RNA Dimers of Cymbidium Ringspot Tombusvirus</title>
<author><name sortKey="Dalmay, Tamas" sort="Dalmay, Tamas" uniqKey="Dalmay T" first="Tamás" last="Dalmay">Tamás Dalmay</name>
<affiliation wicri:level="1"><country xml:lang="fr">Hongrie</country>
<wicri:regionArea>Agricultural Biotechnology Center, Plant Science Institute, Post Office Box 170, 2101 Gödöllö</wicri:regionArea>
<wicri:noRegion>2101 Gödöllö</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Szittya, Gyorgy" sort="Szittya, Gyorgy" uniqKey="Szittya G" first="György" last="Szittya">György Szittya</name>
<affiliation wicri:level="1"><country xml:lang="fr">Hongrie</country>
<wicri:regionArea>Agricultural Biotechnology Center, Plant Science Institute, Post Office Box 170, 2101 Gödöllö</wicri:regionArea>
<wicri:noRegion>2101 Gödöllö</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Burgyan, J Zsef" sort="Burgyan, J Zsef" uniqKey="Burgyan J" first="J Zsef" last="Burgyán">J Zsef Burgyán</name>
<affiliation wicri:level="1"><country xml:lang="fr">Hongrie</country>
<wicri:regionArea>Agricultural Biotechnology Center, Plant Science Institute, Post Office Box 170, 2101 Gödöllö</wicri:regionArea>
<wicri:noRegion>2101 Gödöllö</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j">Virology</title>
<title level="j" type="abbrev">YVIRO</title>
<idno type="ISSN">0042-6822</idno>
<imprint><publisher>ELSEVIER</publisher>
<date type="published" when="1995">1995</date>
<biblScope unit="volume">207</biblScope>
<biblScope unit="issue">2</biblScope>
<biblScope unit="page" from="510">510</biblScope>
<biblScope unit="page" to="517">517</biblScope>
</imprint>
<idno type="ISSN">0042-6822</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0042-6822</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
<langUsage><language ident="en">en</language>
</langUsage>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Abstract: Inoculation of Nicotiana clevelandii and N. benthamiana plants with in vitro transcripts of both genomic and defective interfering (DI) RNAs of cymbidium ringspot tombusvirus resulted in a rapid accumulation of new DI-like RNA species which were demonstrated by cloning and sequencing to be head-to-tail dimers of unit length DI RNAs. The junction regions of dimers were represented by sequences derived precisely from the 5′ and 3′ termini of DI RNAs. Only infection with DI RNAs of smaller size (DI-2 and DI-3, 402 and 482 nt, respectively) produced detectable amount of dimers; in contrast, infection with the largest DI RNA (DI-13, 679 nt) was unable to accumulate dimers during viral infection. Analysis of mutant DI RNAs containing deletions or insertions revealed that the size of the monomer molecule is a major factor in the accumulation of dimers. Monomeric DI RNAs were formed in both plants and protoplasts inoculated with in vitro-transcribed directs. No heterodimers were found in plants inoculated simultaneously with DI-2 and DI-3 RNA molecules, which may indicate that replicase is not released from the template during synthesis of dimer molecules. However, the occurrence of a recombinant DI RNA dimer molecule derived from the two DI RNAs suggests that simultaneous infection of the same cells with two DI RNAs did indeed take place and that absence of heterodimers did not depend on compartmentalization.</div>
</front>
</TEI>
<affiliations><list><country><li>Hongrie</li>
</country>
</list>
<tree><country name="Hongrie"><noRegion><name sortKey="Dalmay, Tamas" sort="Dalmay, Tamas" uniqKey="Dalmay T" first="Tamás" last="Dalmay">Tamás Dalmay</name>
</noRegion>
<name sortKey="Burgyan, J Zsef" sort="Burgyan, J Zsef" uniqKey="Burgyan J" first="J Zsef" last="Burgyán">J Zsef Burgyán</name>
<name sortKey="Szittya, Gyorgy" sort="Szittya, Gyorgy" uniqKey="Szittya G" first="György" last="Szittya">György Szittya</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004180 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004180 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:4F7A384318A00A102D81113FBDC4101FF1F267F9 |texte= Generation of Defective Interfering RNA Dimers of Cymbidium Ringspot Tombusvirus }}
This area was generated with Dilib version V0.6.33. |